The free-living amoebae are a group of organisms that are distributed around the world and commonly inhabit different aqueous media. Medical importance for the human being are among others, Acanthamoeba spp. and Naegleria fowleri. Acanthamoeba castellanii may cause infection in the skin and lung, as well as occasionally granulomatous encephalitis and is the etiologic agent responsible for eye infection, amoebic keratitis of the cornea that not treated on time and properly puts at risk the patient view. Naegleria fowleri is the causal agent of almost always fatal acute infection, primary amoebic meningoencephalitis that occasionally infects the human and other mammals. Currently, we studied some aspects of these amoeba cell biology, such as the comparative study of the mechanisms of pathogenicity on monolayers of MDCK cells, corneas of hamster and ultrastructural characterization of their surface by means of specific markers. In addition, studies are done to carry out the morphological identification and extent of in vitro pathogenicity of amoebae isolated from clinical cases related to amoebic keratitis.

Classical publications

• Mora-Galindo, J., Anaya-Velázquez, F.; Ramírez-Romo, S. and González-Robles, A. Entamoeba histolytica: correlation of assessment methods to measure erythrocyte digestión, and effect of cysteine proteinases inhibitors in HM-1:IMSS and HK-9:NIH strains. Experimental Parasitology. 108 89-100, 2004.
• González Lázaro Mónica., González Robles, A., Hernández Gutiérrez, Rodolfo y Arroyo Verástegui, R. The HeLa cell nucleous, a possible nucleotide source for Trichomonas vaginalis. The international Journal of Biochemistry & Cell Biology. 37 166-176, 2005.
• Chávez Munguía, B., Omaña Molina, M., González Lázaro, M., González Robles, A., Bonilla, P. y Martínez Palomo, A. Ultrastructural study of encystation and excystation in Acanthamoeba castellanii.J Eukariot Microbiol 52 (2) 153-158, 2005.
• Moreno Brito, V., Yáñez Gómez, C., Meza Cervantes, P., Ávila González, L., Rodríguez, M.A., Ortega López, J., González Robles, A. y Arroyo, R. A.Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome piruvate: ferredoxin oxidoreductase is a surface adhesin induced by iron. Cellular Microbiology 7 (2) 245-258, 2005.
• Hernández-Quintero, M., Kuri-Harcuch, W., González-Robles, Arturo, Castro-MuñozLedo, Federico. Interleukin-6 promotes human epidermal keratinocyte proliferation and keratin cytoskeleton reorganization in culture. Cell & Tissue Research. - En prensa –
• González Robles, A., Castañón, G., Cristóbal Ramos, A.R., Lázaro Haller, A., Omaña Molina, M., Bonilla, P. and Martínez Palomo, A. Acanthamoeba castellanii: Structural basis of the cytophatic mechanisms. Experimental Parasitology.– En prensa -
• Martínez Palomo, A., González Robles, A. and De la Torre M. Selective agglutination of patogenic strains of Entamoeba histolytica induced by Concanavalin A. Nature New Biology. 245: 186-187, 1973.
• Arroyo, R., González-Robles, A., Martínez-Palomo, A.and Alderete, J.F.Signalling of Trichomonas vaginalis for amoeboid transformation and adhesin synthesis follows cytoadherence. J.Mol. Microbiol. 7: (2), 299-309, 1993.
• González Lázaro Mónica., González Robles, A., Hernández Gutiérrez, Rodolfo and Arroyo Verástegui, R. HeLa cell nucleous, a possible nucleotide source for Trichomonas vaginalis. The international Journal of Biochemistry & Cell Biology. 37 166-176, 2005.
• González Robles, A., Castañón, G., Cristóbal Ramos, A.R., Lázaro Haller, A., Omaña Molina, M., Bonilla, P. and Martínez Palomo, A. Acanthamoeba castellanii: Structural basis of the cytophatic mechanisms. Experimental Parasitology. 114: 133-140, 2006
• .González Robles, A., Castañón, G., Hernández-Ramírez, V. I., Salazar Villatoro, L., González Lázaro, M., Omaña Molina, M., Talamás Rohana, P. and Martínez Palomo, A. Acanthamoeba castellanii: Identification and distribution of actin cytoskeleton. Experimental Parasitology. 119: 411-417, 2008.
• González Robles, A., González Lázaro, M., Omaña Molina, M., Martínez Palomo, A. Acanthamoeba castellanii: endocytic structures involved in the ingestión of diverse target elements. Acta Protozoologica. 48: 327-332, 2009.
• Omaña Molina, M., González Robles, A., Salazar Villatoro L. I., Cristóbal Ramos A. R., González Lázaro, M., Salinas Moreno, E., Méndez Cruz, R., Sánchez Cornejo, M., De la Torre, González E., Martínez Palomo, A. Acanthamoeba castellanii: Morphological analysis of the interaction with human cornea. Experimental parasitology. 126 73-78, 2010.