Arroyo Verástegui Rossana PhD

Dra. Rossana Arroyo Verátegui

Arroyo Verástegui Rossana PhD

Current position: Full Professor 3D
Present adscription: Department of Infectomics and Molecular Pathogenesis. Cinvestav, IPN, Mexico.       
Address: Av. IPN, No. 2508, San Pedro Zacatenco. CP. 07360
Phone number: +52 (55) 5747 3376
E-mail: rarroyo@cinvestav.mx
Academic Profile

B.Sc. Chemist-Pharmaceutic-Biologist, Universidad de Guadalajara, 1981
Ph.D. Molecular Biology, Cinvestav, 1987

Member of the National System of Researchers (SNI): Level III

Our group studies 1) Molecular pathogenesis of trichomoniasis, particularly adherence, cytotoxicity/apoptosis and hemolysis events. This has allowed us to identify and characterize some cysteine proteinase (CPs), endogenous CPs inhibitors (tricocystatins), aspartic proteases and multifunctional proteins such as adhesins and receptors for extracellular matrix (metabolic enzymes) as virulence factors of Trichomonas vaginalis. 2) The participation of environmental factors such as iron and glucose in gene expression regulation. We have identified an atypical iron-mediated post-transcriptional regulation mechanism involving atypical RNA-binding proteins as multifunctional cytoskeleton and heat shock proteins. 3) Unconventional mechanisms for the transport of vesicles and autophagy of the hydrogenosome (hydrogenophagy) as part of the mechanisms of relocation of multifunctional proteins to the parasite membrane. 4) Recombinant virulence factors as potential targets for diagnosis. 5) We also have a collaborative work in search of therapeutic targets for treatment and vaccine of the T. vaginalis infection.

1. Arroyo R and Orozco E. 1987. Localization and identification of Entamoeba histolytica adhesin. Mol. Bioch. Parasitol. 23:151-158.
Adhesinas de Trichomonas vaginalis como proteínas “moonlighting”

2. Arroyo R, Engbring J, and Alderete JF. 1992. Molecular basis of host epithelial cell recognition by Trichomonas vaginalis. Molec. Microbiol. 6:853-862.

3. Arroyo R, González Robles A, Martínez Palomo A, and Alderete JF. 1993. Signalling of Trichomonas vaginalis for amoeboid transformation and adhesin synthesis follows cytoadherence. Molec. Microbiol. 7:299-310.

4. Moreno-Brito V, Yañez-Gómez C, Meza-Cervántez P, Avila-González L, Rodríguez-Rodríguez MA, Ortega-López J, González-Robles A and Arroyo R. 2005. A Trichomonas vaginalis 120-kDa protein with identity to hydrogenosome pyruvate ferredoxin oxidorreductase is a surface adhesin induced by iron. Cell. Microbiol. 7: 245-258.

5. Meza-Cervantez P, González-Robles A, Cárdenas-Guerra RE, Ortega-López J, Saavedra E, Pineda E, and Arroyo R. 2011. Pyruvate:ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells. Microbiology (UK) 157: 3469-3482.

6. Hernández-García MS, Jesus F. T. Miranda-Ozuna, Lizbeth Salazar-Villatoro, Carlos Vázquez-Calzada, Leticia Avila-Gonzalez, Arturo Gonzalez-Robles, Ortega-López and Rossana Arroyo. 2019. Biogenesis of Autophagosome in Trichomonas vaginalis during Macroautophagy Induced by Rapamycin-treatment and Iron or Glucose Starvation Conditions. J Euk Microbiol. 8. doi: 10.1111/jeu.12712. ISSN electrónico: 1550-7408.
Proteasas e inhibidores de CPs en la virulencia (adhesión, citotoxicidad, hemólisis) de Trichomonas vaginalis.

7. Arroyo R and Alderete JF. 1989. Trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. Infect. Immun. 57 (10):2991-2997.

8. Alvarez-Sánchez ME, Avila-González L, Becerril-García C, Fattel-Facenda LV, Ortega-Lopez J and Arroyo R. 2000. A novel cysteine proteinase (CP65) of Trichomonas vaginalis involved in cytotoxicity. Microb. Path. 28: 193-202.

9. Mendoza-López MR, Becerril-García C, Fattel-Facenda LV, Avila-González L, Ruiz-Tachiquín ME, Ortega-López J and Arroyo R. 2000. CP30: A cysteine proteinase involved in Trichomonas vaginalis cytoadherence. Infect. Immun. 68 (9):4907-4912.

10. León-Félix J, Ortega-López J, Orozco-Solís R and Arroyo R. 2004. Two novel asparaginyl endopeptidase-like cysteine proteinases from the protist Trichomonas vaginalis: their evolutionary relationship within the clan CD cysteine proteinases. Gene 335: 25-35.

11. Hernández-Gutiérrez R, Avila-González L, Ortega-López J, Cruz-Talonia F, Gómez-Gutiérrez G and Arroyo R. 2004. Trichomonas vaginalis: characterization of a 39-kDa cysteine proteinase found in patient vaginal secretions. Exp. Parasitol. 107: 125-135.

12. Ramón-Luing LA, Rendón-Gandarilla FJ, Puente-Rivera J, Avila-González L and Arroyo R. 2011. Identification and characterization of the immunogenic cytotoxic TvCP39 proteinase gene of Trichomonas vaginalis. Internal. J. Biochem. Cell Biol. 43: 1500–1511.

13. Rendón-Gandarilla FJ, Ramón-Luing LA, Ortega-López J, de Andrade IR, Benchimol M, Arroyo R. 2013. The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence. BioMed Res Internal. 2013:561979. 18 pages, Doi:10.1155/2013/561979.

14. Cárdenas-Guerra RE, Arroyo R, de Andrade IR, Benchimol M and Ortega-López J. 2013. Iron-induced cysteine proteinase TvCP4 plays a key role in Trichomonas vaginalis haemolysis. Microb. Infect. 15: 959-968.

15. Cárdenas-Guerra RE, Arroyo R and Ortega-López J. 2015. The recombinant TvCP4 pre-pro region is an inhibitor of cathepsin-L proteolytic activity in Trichomonas vaginalis. Internal. J. Biochem. Cell Biol. 59: 73-83.

16. Puente-Rivera J, Ramón-Luing LA, Figueroa-Angulo EE, Ortega-López J, and Arroyo R. 2014. Trichocystatin-2 (TC-2): an endogenous inhibitor of cysteine proteinases in Trichomonas vaginalis is associated with TvCP39. Internal. J. Biochem. Cell Biol. 54C: 255-265.

17. Resendiz-Cardiel, Arroyo R, Ortega-López J. 2017. Expression of the enzymatically active legumain-like cysteine proteinase TvLEGU-1 of Trichomonas vaginalis in Pichia pastoris. Protein Expression and Purification. 134:104-113. doi: 10.1016/j.pep.2017.04.007. ISSN 1046-5928.

18. Sánchez-Rodríguez Diana Belén, Ortega-López Jaime, Cárdenas-Guerra Rosa Elena, Reséndiz-Cardiel Gerardo, Chávez-Munguía Bibiana, Lagunes-Guillén Anel, Arroyo Rossana. 2018. Characterization of a novel endogenous cysteine proteinase inhibitor, trichocystatin-3 (TC-3), localized on the surface of Trichomonas vaginalis. Int. J. Biochem. Cell Biol. 102: 87-100.
Efecto de hierro en la expresión de factores de virulencia y mecanismos de regulación posttranscripcional en T. vaginalis.

19. Lehker WM, Arroyo R, and Alderete JF. 1991. The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis. J. Exp. Med. 174:311-318.

20. Solano-González E, Burrola-Barraza E, León-Sicairos CR, Avila-Gónzalez L, Gutierrez-Escolano L, Ortega-López J and Arroyo R. 2007. The trichomonad cysteine proteinase TVCP4 transcript contains an iron-responsive element. FEBS LETTERS 581: 2919-2928.

21. Torres-Romero JC and Arroyo R. 2009. Responsiveness of Trichomonas vaginalis to iron concentrations: Evidence for a post-transcriptional iron regulation by an IRE/IRP-like system. Infect. Genet. Evol. 9: 1065-1074.

22. Calla-Choque JS, Figueroa-Angulo EE, Avila-González L, and Arroyo R. 2014. a-Actinin TvACTN3 of Trichomonas vaginalis is an RNA-binding protein that may participate in its post-transcriptional iron regulatory mechanism. BioMed Res. Internal. 2014:424767 20 pages (doi.org 10.1155/2014/424767).

23. Figueroa-Angulo EE, Calla-Choque JS, Mancilla-Olea MI and Arroyo R. 2015. RNA-binding proteins in Trichomonas vaginalis: Atypical multifunctional proteins involved in a posttranscriptional iron regulatory mechanism. Biomolecules special issue in “RNA-Binding Proteins—Structure, Function, Networks and Disease”. Biomolecules, 5:3354-3395. Doi:10.3390/biom5043354.

24. Arroyo R, Cárdenas-Guerra RE, Figueroa-Angulo EE, Puente-Rivera J, Zamudio-Prieto O and Ortega-López J. 2015. Trichomonas vaginalis cysteine proteinases: Iron response in gene expression and proteolytic activity. BioMed Res. Internal. Special Issue “Iron and Parasites. 2015: 946787. 20 pages. DOI: 10.1155/2015/946787.

25. Rivera-Rivas LA, Lorenzo-Benito S, Sánchez-Rodríguez DB, Miranda-Ozuna JFT, Euceda-Padilla EA, Ortega-López J, Chávez-Munguía B, Lagunes-Guillén A, Velázquez-Valassi B, Jasso-Villazul L, Arroyo R. 2020. The effect of iron on Trichomonas vaginalis TvCP2, a cysteine proteinase found in vaginal secretions of trichomoniasis patients. Parasitology (UK). 147:760-774. https://doi.org/ 10.1017/S0031182020000438. ISSN impreso 0031-1820; electrónico: 1469-8161.
Efecto de la glucosa en la virulencia de Trichomonas vaginalis.

26. Figueroa-Angulo EE, Rendón-Gandarilla FJ, Puente-Rivera J, Calla-Choque JS, Cárdenas-Guerra RE, Ortega-López J, Quintas-Granados LI, Alvarez-Sánchez ME, Arroyo R. 2012, The effects of environmental factors on the virulence of Trichomonas vaginalis. Microb. Infect. 14 (15):1411-1427.

27. Figueroa-Angulo EE, Estrella-Hernández P, Salgado-Lugo H, Ochoa-Leyva A, Gómez Puyou A, Campos SS, Montero-Moran G, Ortega-López J, Saab-Rincon G, Arroyo R, Benítez-Cardoza CG and Brieba LG. 2012. Cellular and biochemical characterization of two closely related Triosephosphate isomerases from Trichomonas vaginalis. Parasitology 139 (13): 1729-1738.

28. Mancilla-Olea MI, Ortega-López Jaime, Figueroa-Angulo Elisa E., Avila-González Leticia, Cárdenas-Guerra Rosa Elena, Miranda-Ozuna Jesús F.T., González-Robles Arturo, Hernández-García Mar Saraí, Sánchez-Ayala Lizbeth, Arroyo Rossana. 2018. Trichomonas vaginalis cathepsin D-like aspartic proteinase (Tv-CatD) is positively regulated by glucose and degrades human hemoglobin. Int J Biochem Cell Biol. 97: 1-15. doi: 10.1016/j.biocel.2018.01.015. ISSN 1357-2725.

29. Miranda-Ozuna JFT, Hernández-García MS, Brieba LG; Benítez-Cardoza CG, Ortega-López J, González-Robles A, Arroyo R. 2016. The Glycolytic Enzyme Triosephosphate Isomerase of Trichomonas vaginalis (TvTIM) Is a Surface-Associated Protein Induced by Glucose that functions as a Laminin and Fibronectin-Binding Protein. Infect. Immun. 84 (10): 2878-2894.

30. Miranda-Ozuna JFT, Luis Alberto Rivera-Rivas, Rosa Elena Cárdenas-Guerra, Mar Saraí Hernández-García, Bibiana Chávez-Munguía, Arturo González-Robles and Rossana Arroyo. 2019. Glucose-restriction increases Trichomonas vaginalis cellular damage towards HeLa cells and proteolytic activity of cysteine proteinases (CP), such as TvCP2. Parasitology (UK). doi.org/10.1017/S0031182019000209.
Biomarcadores para diagnóstico y tratamiento de la tricomonosis

31. Ramón-Luing LA, Rendón-Gandarilla FJ, Cárdenas-Guerra RE, Rodríguez-Cabrera NA, Ortega-López J, Ávila-González L, Ángel-Ortiz C, Herrera-Sánchez CN, Mendoza-García M and Arroyo R. 2010. Immunoproteomics of the active degradome to identify biomarkers for Trichomonas vaginalis. Proteomics 10 (3): 435-444.

Citas en artículos de investigación s/autocitas: >2000.

1. Arroyo Verástegui Rossana. Cisteína proteinasas útiles para el diagnóstico de la infección con Trichomonas vaginalis (presentada al IMPI el 24 de julio, 2006). Otorgada 28 de mayo, 2015. Patente Mexicana Título No. 330574.

2. Arroyo Verástegui Rossana y Ortega López Jaime. Proteína citotóxica de Trichomonas vaginalis (presentada al IMPI el 14 de septiembre, 2006). Otorgada 13 de diciembre, 2012. Título No. 307902.

3. Arroyo Verástegui Rossana. “La proteína adhesina piruvato ferredoxín-óxidoreductasa (pfo) como blanco para inhibición de adherencia del parásito Trichomonas vaginalis y como blanco diagnóstico y vacunal para la tricomonosis”. No. de expediente MX/a/2011/011361. Otorgada 09/03/2016. Patente Mexicana Título No. 337629.

4. Arroyo Verástegui Rossana, Rendón-Gandarilla Francisco Javier, Resendiz Cardiel Gerardo y Ortega-López Jaime. “Cisteína proteinasa TvLEGU-1 como blanco terapéutico en la infección por Trichomonas vaginalis”. No. de expediente MX/a/2012/011701. Otorgada 08/03/2018. Patente Mexicana Título No. 354619.

5. Arroyo Verástegui Rossana.” Métodos diagnósticos y terapéuticos para tratamiento de la infección por Trichomonas vaginalis mediante la proteína TvCP39“. No. de expediente: MX/a/2011/007531. Otorgada 13/11/2018. Patente Mexicana Título No. 361196.

1. Ganadora del premio UNESCO-GUINEA ECUATORIAL 2012. Por las aportaciones en las ciencias de la vida para mejorar la calidad de vida del humano. 17/07/12. UNESCO, Paris, Francia.

2. Visitante distinguida. H. Ayuntamiento de Veracruz, Ver. Por la participación en las Jornadas Científicas Franco-Mexicanas sobre Enfermedades Infecciosas, mayo, 2010. Veracruz, Ver.

3. Editor asociado de la revista JOMICS a partir del 2010.

4. Primer lugar en el trabajo: “Verificación del uso de las cisteín proteinasas recombinantes TvCP39r y TvCP4r en ensayos tipo ELISA para el diagnóstico de la tricomonosis” presentado en la Sección de Ciudad Saludable, Salud Sexual y Reproductiva durante la Semana de la Ciencia y la Innovación 2010, Ciudad de México, 22 al 26 de noviembre, 2010. Medalla de Plata.

5. Invitaciones como conferencista en reuniones o congresos científicos de prestigio: 62.

6. Premio a la estudiante M. en C. Sarahí Rodríguez Cruz. Fotomicrografía ganadora concurso Infectious Diseases Hub, 2019.