We are currently studying three protozoan parasite, Entamoeba histolytica, Trypanosoma cruzi and Leishmania mexicana. These unicellular organisms are pathogenic to humans and they are the causative agents of amebiasis, Chagas disease and leishmanisis respectively. The main areas of study in which the laboratory is engaged are: (i) Development and implementing diagnostic methods, (ii) Identification of genes encoding parasite surface proteins involved in the interaction with the host and genes that codify parasite stage-specific antigens, (iii) Role of protein tyrosine phosphatases in the parasite biology and in the pathogenic mechanisms, (iv) Cellular and humoral immune response against native and recombinant antigens, and (v) Vaccines development.

 1      Olivas-Rubio, M., Hernández-Martínez, S., Talamás-Rohana, P., Tsutsumi, V., Reyes-López, P., Rosales-Encina J.L..  cDNA cloning and partial characterization of amastigote specific surface glycoprotein from Trypanosoma cruzi.  Infect. Gen. Evol., 9, 2009, pp. 1083-1091. 

2      Alejandro Carabarin-Lima, María Cristina González-Vázquez, Baylon-Pacheco Lidia, Patricia-Talamas Rohana and Rosales-Encina José Luis. Immunization with the Recombinant Surface Protein rTcSP2 Alone or Fused to the CHP or ATPase Domain of TcHSP70 Induces Protection Against Acute Trypanosoma cruzi Infection. (2010). Journal of Vaccines &Vaccination 1:110.

3      Minerva Arce-Fonseca, Angel Ramos-Ligonio, Aracely López-Monteón, Berenice Salgado- Jiménez, Patricia Talamás-Rohana, and José Luis Rosales-Encina.  A DNA vaccine encoding for TcSSP4 induces protection against acute and chronic infection in experimental Chagas disease. International Journal of Biological Sciences. 2011; 7(9):1230-1238.

4      Yevel Flores-García, Jose Luis Rosales-Encina, Abhay R. Satoskar and Patricia  Talamás-Rohana. IL-10-IFN-γ double producers CD4+ T cells are induced by immunization with an amastigote stage specific derived recombinant protein of Trypanosoma cruzi. International journal of biological sciences 2011;7(8):1093-1100. 

5      María Cristina González-Vázquez, Alejandro Carabarin-Lima, Lidia Baylon-Pacheco, Patricia Talamás-Rohana, José Luis Rosales-Encina. Obtaining of three recombinant antigens of Entamoeba histolytica and evaluation of their immunogenic ability without adjuvant in a hamster model of immunoprotection. Acta Trop. 2012 122(2):169-76.

6      Yadira Morán-Utrera, Aracely López-Monteon, José Luis Rosales-Encina, Enrique Méndez-Bolaina and Angel Ramos-Ligonio. Trypanosoma cruzi SSP4 amastigote protein induces expression of immunoregulatory and immunosuppressive molecules in peripheral blood mononuclear cells. Journal of Tropical Medicine. Volume 2012, Article ID 829139, 10 pages. doi:10.1155/2012/829139 [ISSN: 1687-9686 (Print), ISSN: 1687-9694 (Online), doi:10.1155/JTM].

7      Minerva Arce-Fonseca, Olivia Rodríguez-Morales, M Magdalena Pérez-Leyva, Martha A Ballinas-Verdugo, Silvia C Carrillo-Sánchez, José Luis Rosales-Encina, Ricardo Alejandre-Aguilar, Pedro A Reyes (2012). Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine model. Veterinary Research 43:79.

 8      Laura Mónica Álvarez-Rodríguez, Angel Ramos-Ligonio, José Luis Rosales-Encina, María Teresa Martínez-Cázares, Aurora Parissi-Crivelli and Aracely López-Monteon (2012). Expression, purification and evaluation of diagnostic potential and immunogenicity of a recombinant NS3 protein from all serotypes of dengue virus. Journal of Tropical Medicine, Article ID 956875, 10 pages, doi:10.1155/2012/956875

 9      Berenice Salgado-Jiménez, Minerva Arce-Fonseca, Lidia Baylón-Pacheco, Patricia  Talamás-Rohana, José Luis Rosales-Encina. Differential immune response in a murine Chagas disease model immunized either with SPA, SPR, or SPC domains from TcSP protein of Trypanosoma cruzi or with the corresponding coding DNAs , Parasite immunology 2013, 35:32-41.

 10   Yevel Flores-García, José Luis Rosales-Encina, Víctor Hugo Rosales-García, Abhay Satoskar and Patricia Talamás-Rohana. CD4+CD25+FOXP3+ Treg cells induced by rSSP4, derived from T. cruzi amastigotes, increase parasitemia in an experimental Chagas disease model. Volume 2013 (2013), Article ID 632436, 10 pages.