Rodríguez Mario Alberto PhD

Dr. Mario Alberto Rodríguez Present position: Professor
Present adscription: Department of Infectomics and Molecular Pathogenesis. Center for Research and Advanced Studies, National Polytechnical Institute, Mexico.       
Address: Av. IPN, No. 2508, San Pedro Zacatenco. CP. 07360
Phone number: + 52 (55) 57-47-3800 ext 5653 and 5680. Fax: + 52 (55) 57-47-3800 ext 5671
E-mail: marodri@cinvestav.mx

Academic profile

Undergraduate studies: Biologist: FES Iztacala, Universidad Nacional Autónoma de México. México. 1985.
Mr. Sc.:  Molecular Biology. Centro de Investigación y de Estudios Avanzados del IPN. Mexico City, México. 1986.
Ph. D. Molecular Biology. Centro de Investigación y de Estudios Avanzados del IPN. Mexico City, México. 1990.
 

Info

Our interest is to study the protozoan Entamoeba histolytica. This parasite causes amoebic dysentery and amebic liver abscess, which are associated with significant morbidity and mortality throughout the world, especially in developing countries. In our laboratory we identify and characterize several molecules that participate in survival and/or in the pathogenic mechanism of this parasite, among these are: a) the adhesin and the protease that form the EhCPADH complex, which is involved in adhesion and invasion of the host tissues; (b) The EhRabB protein, a small GTPase that is involved in the phagocytosis of the target cells; and (c) ion channels and ionic pumps involved in cellular homeostasis. In addition, we are interested in studying the mechanisms involved in the regulation of gene expression in this microorganism, identifying and characterizing both transcriptional factors and enzymes involved in the epigenetic regulation.

On the other hand, we are also interested in studying odontogenic tumors, neoplasms which derive from the forming tissues of the dental organ and its remaining structures. The reasons of the molecular pathogenesis and the peculiar biological behavior of the different types of odontogenic tumors do not have been clearly elucidated, despite the high rate of recurrence and the possibility of metastasis. In the laboratory we carry out proteomics studies on two types of odontogenic tumors: ameloblastoma, from epithelial origin and odontogenic myxoma, from mesenchymal origin. In addition, cell cultures of these and other odontogenic lesions are being obtained to establish in vitro systems that allow a greater advance in the study of these pathologies.

  1. Rodríguez, M.A. and Orozco E. (1986). Isolation and characterization of phagocytosis- and virulence-deficient mutants of Entamoeba histolytica. J. Infect. Dis. 154: 27-32.

  2. Rodríguez M.A., Hernández F., Santos L., Valdez A. and Orozco E. (1989). Entamoeba histolytica: molecules involved in the target cell-parasite relationship. Mol. Biochem. Parasitol. 37: 87-99.

  3. Rodríguez M.A., Baéz-Camargo M., Delgadillo D. M. and Orozco E. (1996). Cloning and expression of an Entamoeba histolyitica NADP+-dependent alcohol dehydrogenase gene. Biochim. Biophys. Acta. 1306: 23-26.

  4. Rodríguez M.A., Hidalgo M.E., Sánchez T. and Orozco E. (1996). Cloning and characterization of the Entamoeba histolytica pyruvate: ferredoxin oxidoreductase gene. Mol. Biochem. Parasitol. 78: 273-277.

  5. Rodríguez M.A., García-Pérez R. M. and Orozco E. 1997. Subcellular location of the pyruvate:ferredoxin oxidoreductase from Entamoeba histolytica. Arch. Med. Res. 28: S89-S90.

  6. Rodríguez M.A., García-Pérez R. M., Sánchez T, Guillen N., Mendoza L., Orozco E. 1998. The pyruvate:ferredoxin oxidoreductase is located in the plasma membrane and in a cytoplasmic structure in Entamoeba. Microb Path 25:1-10.

  7. García-Rivera G, Rodríguez M.A., Ocádiz R., Martínez-López M..C., Arroyo R., González-Robles A. and Orozco E. 1999. Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112 kDa surface protein. Mol Microbiol 33: 556-568.

  8. Rodríguez M.A. García-Pérez RM, García-Rivera G, López-Reyes I, Mendoza L, Ortiz-Navarrete V, Orozco E. 2000. An Entamoeba histolytica Rab-like encoding gene and protein: Function and cellular location. Mol Biochem Parasitol 108: 199-206.

  9. Rodriguez M.A., Orozco E. 2000.Characterization of the EhRabB recombinant protein of Entamoeba histolytica. Arch Med Res. 31(4 Suppl):S171-172.

  10. García-Rivera G, Gómez C, Pérez DG, Flores E, Rodríguez M.A., Orozco E. 2000. Structural characterization of the 5'-flanking regions of the Entamoeba histolytica Ehcp112 and Ehadh112 genes. Arch Med Res. 31(4 Suppl):S303-304.

  11. Solís F, Orozco E, Córdova L, Rivera B, Luna-Arias JP, Gómez-Conde E, Rodríguez M.A. 2002. Entamoeba histolytica: DNA carrier vesicles in nuclei and kinetoplast-like organelles (EhkOs). Mol Gen Genom. 267: 622-628.

  12. Mendoza L, Orozco E, Rodríguez M.A., García-Rivera G, Sánchez T, García E, Gariglio G. 2003. Ehp53, an Entamoeba histolytica protein, ancestor of the mammalian tumor suppressor p53. Microbiology 149: 885-893.

  13. 14. Martínez-López C, Orozco E, Sánchez T, García-Pérez RM, Hernández-Hernández F, Rodríguez M.A. 2004. The EhADH112 recombinant polypeptide inhibits cell liver abscesses formation by Entamoeba histolytica trophozoites. Cell Microbiol 6: 367-376.

  14. Madriz X, Martínez MB, Rodríguez M.A., Sierra G, Martínez-López C, Riverón AM, Flores L, Orozco E. 2004. Expression in fibroblast and in live animals of Entamoeba histolytica EhCP112 and EhADH112 polypeptides. Microbiology 150: 1251-1260.

  15. Ocádiz R, Orozco E, Carrillo E, Quintas LI, Ortega-López J; García-Pérez RM, Sánchez T, Castillo-Juárez BA, García-Rivera G, Rodríguez M.A. 2005. EhCP112 is an Entamoeba histolytica secreted cysteine protease that may be involved in the parasite-virulence. Cell Microbiol 7: 221-232.

  16. Picazarri K, Luna-Arias JP, Carrillo E, Orozco E, Rodríguez M.A. 2005. Entamoeba histolytica: Identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB. Exp Parasitol 110: 253-258.

  17. Salas-Casas A, Ponce-Balderas A, García-Pérez RM, Cortes.-Reynosa P, Gamba G, Orozco E, Rodríguez M.A. 2006. Identification and functional characterization of EhClC-A, an Entamoeba histolytica ClC chloride channel located at plasma membrane. Mol Microbiol 59: 1249-1261.

  18. Romero-Díaz M, Gómez C, López-Reyes I, Orozco E, Rodríguez M.A.. 2007. Structural and functional analysis of the Entamoeba histolytica EhrabB gene promoter. BMC Molecular Biology 8: 82. doi:10.1186/1471-2199-8-82.

  19. 20. Martínez MB, Rodríguez M.A., García-Rivera G, Sánchez T, Hernández-Pando R, Aguilar D, Orozco E. 2009. A pcDNA-Ehcpadh vaccine against Entamoeba histolytica elicts a protective Th1-like response in hamster liver. Vaccine 27:4176-4186.

  20. Gomez C, Ramirez ME, Calixto-Galvez M, Medel O, Rodríguez M.A.. 2010. Regulation of gene expression in protozoa parasites. J Biomed Biotechnol 2010: 726045.

  21. Calixto-Galvez M, Romero-Díaz M, García-Muñoz A, Salas-Casas A, Pais-Morales J, Galván IJ, Orozco E, Rodríguez M.A.. 2011. Identification of a polypeptide containing Tudor and Staphyloccocal nuclease-like domains as the sequence-specific binding protein to the upstream regulatory element 1 of Entamoeba histolytica. Int J Parasitol 41:775-782.

  22. García-Muñoz A, Rodríguez M.A., Bologna-Molina R, Cázares-Raga FE, Hernández-Hernández FC, Farfán-Morales JE, Trujillo JJ, Licéaga-Escalera C, Mendoz-Hernández G. 2012. The orosomucoid 1 protein (a1 acid glycoprotein) is overexpressed in odontogenic myxoma. Proteome Sci 10:49.

  23. Guzmán-Medrano R, Arreola-Rosales RL, Shibayama M, Silva-Olivares DA, Bologna-Molina R, Rodríguez M.A. 2012. Tumor associated macrophages and angiogenesis: a statistical correlation that could reflect a critical relationship in ameloblastoma. Path Res Pract 208: 672-676.

  24. Juárez-Hernández LJ, García-Pérez RM, Salas-Casas A, García-Rivera G, Orozco E, Rodríguez M.A. 2013. Entamoeba histolytica: the over expression of a mutated EhRabB protein produces a decrease of in vitro and in vivo virulence. Exp Parasitol. 133: 339-345.